Composite

Part:BBa_K631037:Design

Designed by: Allister Smith   Group: iGEM11_Queens_Canada   (2011-09-28)

GPC-1:eCFP:UNC-54 (for use in C. elegans)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2814
    Illegal SapI site found at 1764
    Illegal SapI.rc site found at 1596


Design Notes

GPC-1: PCR from genomic (C. elegans) DNA

eCFP: Existing reporter (BBa_K309020)

UNC-54: Existing terminator (BBa_K309012)

Source

Test

References